Swtyblz — Encodes

BLASTx translates the nucleotide sequence in all six reading frames and compares the resulting protein sequences to the NCBI non-redundant (nr) database. This bypasses the corrupted identifier and asks: does this sequence encode a known protein domain?

Locate the raw FASTA, FASTQ, or GenBank file where the identifier appears. Look for a corresponding "swtyblz" in the header comments. Often, the original submitters include a definition line such as >swtyblz [organism=...] [strain=...]. swtyblz encodes

If you have synthesized an mRNA transcript containing the codon sequence for SWTYBLZ (5'-UCC UGG ACC UAC [NNU/GAY] CUG [CAR/GAR]-3'), you will encounter a problem: The ribosome stalls. BLASTx translates the nucleotide sequence in all six

Consequently, SWTYBLZ encodes a fatal frameshift or a stop codon if misread. In fact, if "B" is mistakenly read as "D" (GAU) and "Z" as "E" (GAG), the nested sequence "UGA" appears. UGA is the opal stop codon. Thus, the sequence might actually encode: S-W-T-Y-STOP. Consequently, SWTYBLZ encodes a fatal frameshift or a